The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR goods were digested and cloned right into a pET21a+ vector utilizing NdeI and XhoI restriction enzymes. A degree mutation was introduced by the quick modify https://ashleighb218spp4.thechapblog.com/profile