The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR items had been digested and cloned right into a pET21a+ vector working with NdeI and XhoI restriction enzymes. A point mutation was released by the https://zalmayh554vhs8.illawiki.com/user